Sign-up for our newsletter
MAIN
Event Calendar
Awardee Reports
ABOUT DIACOMP
Citing DiaComp
Contact
Committees
Institutions
Awardee Reports
Publications
Bioinformatics
RESOURCES
Protocols & Methods
Reagents & Resources
Mouse Diet
Breeding Schemes
Validation Criteria
IMPC / KOMP Data
Publications
Bioinformatics
CONTACT
PARTICIPANT AREA
Login
▹
Home
Member Profile
Brian Perrino
Personal Information
Title
Associate Professor
Expertise
Gastro-Intestinal (GI)
Institution
University of Nevada-Reno
ORCID
https://orcid.org/0000-0001-7429-9971
Newsletter?
Not signed up.
Data Summary
Type
Count
Grants/SubContracts
1
Progress Reports
1
Publications
3
Protocols
0
Committees
2
Grants/Applications
Progress Reports
Publications
Presentations
Protocols
Committees
Cellular Regulation of Human Proximal stomach Motor Responses
Gastric motility defects present as functional dyspepsia or gastroparesis and often arise as complications of obesity and diabetes. The prevalence of diabetic gastroparesis is increasing due to the increasing incidence of obesity and type 2 diabetes in the US. The objective of the proposed project is to establish a better understanding of the cellular basis of human gastric fundus motility to develop more extensive and focused investigations utilizing NIH R01 and/or P01 levels of support. This study will provide the first evaluation of Ca2+ sensitization mechanisms in human gastric fundus smooth muscles activated by acetylcholine (ACh) in its physiological role as a neurotransmitter. The proposed studies will detail the mechanisms by which myofilament sensitivity to Ca2+ is increased by cholinergic neurotransmission, define approaches that can further increase Ca2+ sensitivity, and examine the potential role of interstitial cells of Cajal (ICC) in specifying how the cholinergic signal increases Ca2+ sensitivity. These studies will have relevance to the clinical management of diabetic gastroparesis by providing a better understanding of the mechanism by which the novel cholinesterase inhibitor Acotiamide improves gastric motility and clinical symptoms in patients with functional dyspepsia or gastroparesis. The specific aims are: 1.Determine the importance of Ca2+ sensitization mechanisms for gastric fundus smooth muscle contraction by cholinergic neurotransmission. We will compare Rho kinase and protein kinase C activation by cholinergic neurotransmission and bath application of carbachol by measuring MYPT1 and CPI-17 phosphorylation. We will use the cholinesterase inhibitor neostigmine to define conditions that increase acetylcholine levels and MYPT1 and CPI-17 phosphorylation, and potentiate the contractile responses. 2. Evaluate the contribution of loss of ICC function to altered Ca2+ sensitization pathways in human gastric fundus smooth muscles. We will examine how disrupting the activity of interstitial cells of Cajal with the novel ANO1 inhibitor benzbromarone affects the activation of Ca2+ sensitization pathways in fundus smooth muscles by cholinergic neurotransmission.
Progress Reports
Drag a column header and drop it here to group by that column
Application
Complete Date
Report
Options
Cellular Regulation of Human Proximal stomach Motor Responses (Perrino, Brian)
11/3/2015
View Progress Report Document
Annual Reports
No uploaded documents found.
Publication
Altmetrics
Submitted By
PubMed ID
Status
Year: 2019; Items: 1
Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions.
Xie Y, Perrino BA
Analytical biochemistry
, 2019 (577), 1 - 13
Perrino, Brian
30981700
Published
Year: 2018; Items: 1
Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes.
Li W, Sasse KC, Bayguinov Y, Ward SM, Perrino BA
Journal of diabetes research
, 2018 (2018), 8743874
Perrino, Brian
29955616
Published
Year: 2016; Items: 1
Calcium Sensitization Mechanisms in Gastrointestinal Smooth Muscles.
Perrino BA
Journal of neurogastroenterology and motility
, 2016 (22), 213 - 25
Perrino, Brian
26701920
Published
No uploaded documents found.
No protocols found.
Name
Description
Steering Committee
The DiaComp Steering Committee is the governing body of the consortium. The principle function of this committee is to guide the scientific direction of the consortium. This is accomplished by creating various subcommittees necessary to advance the scientific goals and providing guidance to the broader complications research community. Policies for the consortium are developed through consultation with the
External Evaluation Committee
Gastro-Intestinal (GI)
The DiaComp Gastro-Intestinal (GI) Committee has the principal function of furthering the mission of the consortium with regard to diabetic gastro-intestinal (GI) and liver disease
Curation Flag Information
Display Stats
New comment to be added:
Flag Active?
OrderID
Experiment
Species
Status
Measurements
Options
No records to display.
Welcome to the DiaComp Login / Account Request Page.
Email Address:
Password:
Note: Passwords are case-sensitive.
Please save my Email Address on this machine.
Not a member?
If you are a funded DiaComp investigator, a member of an investigator's lab,
or an External Scientific Panel member to the consortium, please
request an account.
Forgot your password?
Enter your Email Address and
click here.
ERROR!
There was a problem with the page:
User Info
User Confirm
Please acknowledge all posters, manuscripts or scientific materials that were generated in part or whole using funds from the Diabetic Complications Consortium(DiaComp) using the following text:
Financial support for this work provided by the NIDDK Diabetic Complications Consortium (RRID:SCR_001415, www.diacomp.org), grants DK076169 and DK115255
Citation text and image have been copied to your clipboard. You may now paste them into your document. Thank you!