Guiming Liu

Personal Information
Title Assistant Professor
Expertise Uropathy
Institution Case Western Reserve
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Data Summary
Grants/SubContracts 1
Progress Reports 1
Publications 8
Protocols 0
Committees 2

Adipose-derived MSCs Treatment of Diabetes and Diabetic Bladder Dysfunction
The overall goals of this application are to examine the time-dependence and potential mechanisms of action of adipose-derived mesenchymal stem cells (aMSCs) in the treatment of diabetes mellitus and diabetic bladder dysfunction (DBD). Novel treatments are required for DBD, one of the most common, costly, and yet understudied complications of diabetes. Based on published research and our preliminary data, we hypothesize that administration of aMSCs can reverse the hyperglycemia in streptozotocin-induced type 1 diabetes in a time-dependent manner, having an effect when sufficient insulin-producing cells still remain, but not after long-term, sustained damage. Moreover, we hypothesize that aMSCs can improve DBD to some extent even when the hyperglycemia is not reversed. We further hypothesize that therapeutic effects of aMSCs can be exerted via a paracrine-mediated mechanism, and that administration of conditioned mediumfrom aMSCs can reverse or improve hyperglycemia and DBD. Finally, we hypothesize that culturing aMSCs with serum from diabetic rats will enhance the therapeutic benefit of the CM compared with culturing aMSCswith regular serum, due to the presence of “tissue damage-related” factors in diabetic rat serum that will stimulate aMSCs to secrete specific “tissue repair” factors. Through the Diabetic Complications ConsortiumPilot & Feasibility funding mechanism we propose to test those hypotheses by conducting the following Specific Aims: 1) To examine the effects of aMSCs on hyperglycemia reversal and DBD at different diabetesstages in STZ-induced diabetic rats. 2) To compare the effects of administration of aMSCs vs. conditioned medium from aMSCs cultured with standard fetal bovine serum, normal rat serum, or diabetic rat serum, onhyperglycemia and DBD in STZ-induced diabetic rats. The results of this study will provide important insights into a potential new therapy for patients with DBD. RELEVANCE

Progress Reports

Annual Reports
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Year: 2016; Items: 1

Functional and morphological alterations of the urinary bladder in type 2 diabetic FVB(db/db) mice.
Wu L, Zhang X, Xiao N, Huang Y, Kavran M, Elrashidy RA, Wang M, Daneshgari F, Liu G
Journal of diabetes and its complications, 2016

Year: 2015; Items: 4

Tissue specific dysregulated protein subnetworks in type 2 diabetic bladder urothelium and detrusor muscle.
Tomechko SE, Liu G, Tao M, Schlatzer D, Powell CT, Gupta S, Chance MR, Daneshgari F
Molecular & cellular proteomics : MCP, 2015
Short-term diabetes- and diuresis-induced alterations of the bladder are mostly reversible in rats.
Xiao N, Huang Y, Kavran M, Elrashidy RA, Liu G
International journal of urology : official journal of the Japanese Urological Association, 2015 (22), 410 - 415
Impaired cytokine expression, neutrophil infiltration, and bacterial clearance in response to urinary tract infection in diabetic mice.
Ozer A, Altuntas CZ, Bicer F, Izgi K, Hultgren SJ, Liu G, Daneshgari F
Pathogens and disease, 2015
Advanced glycation end products facilitate bacterial adherence in urinary tract infection in diabetic mice.
Ozer A, Altuntas CZ, Izgi K, Bicer F, Hultgren SJ, Liu G, Daneshgari F
Pathogens and disease, 2015 (73)

Year: 2014; Items: 1

Diabetic bladder dysfunction.
Liu G, Daneshgari F
Chinese medical journal, 2014 (127), 1357 - 1364

Year: 2013; Items: 2

Roles of polyuria and hyperglycemia in bladder dysfunction in diabetes.
Xiao N, Wang Z, Huang Y, Daneshgari F, Liu G
The Journal of urology, 2013 (189), 1130 - 1136
Impact of diabetes mellitus on bladder uroepithelial cells.
Hanna-Mitchell AT, Ruiz GW, Daneshgari F, Liu G, Apodaca G, Birder LA
American journal of physiology. Regulatory, integrative and comparative physiology, 2013 (304), R84 - R93
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