The Presence and Location of Podocytes in Glomeruli as Affected by Diabetes
Authors Maraszek KE, Santo BA, Yacoub R, Tomaszewski JE, Mohammad I, Worral AM, Sarder P
Submitted By Pinaki Sarder on 11/17/2020
Status Published
Journal Proceedings of SPIE--the International Society for Optical Engineering
Year 2020
Date Published 2/1/2020
Volume : Pages 11320 : Not Specified
PubMed Reference 32362706
Abstract The primary purpose of the kidney, specifically the glomerulus, is filtration.
Filtration is accomplished through the glomerular filtration barrier, which
consists of the fenestrated endothelium, glomerular basement membrane, and
specialized epithelial cells called podocytes. In pathologic states, such as
Diabetes Mellitus (DM) and diabetic kidney disease (DKD), variable glomerular
conditions result in podocyte injury and depletion, followed by progressive
glomerular injury and DKD progression. In this work we quantified glomerulus and
podocyte structural changes in histopathology image data derived from a murine
model of DM. Using a variety of image processing techniques, we studied changes
in podocyte morphology and intra-glomerular distribution across healthy, mild
DM, and DM glomeruli. Our feature analysis provided feature trends which we
believe are reflective of DKD pathology; while glomerular area peaked in mild
DM, average podocyte number and distance from the urinary pole continued to
decrease and increase, respectively, throughout DM. Ultimately, this study aims
to augment the set of quantifiable image biomarkers used for evaluation of DKD
progression in digital pathology, as well as underscore the importance of
engineering biologically-inspired image features.