Single-cell transcriptomics of the mouse kidney reveals potential cellular
targets of kidney disease.
Authors Park J, Shrestha R, Qiu C, Kondo A, Huang S, Werth M, Li M, Barasch J, Suszták K
Submitted By Katalin Susztak on 1/2/2019
Status Published
Journal Science (New York, N.Y.)
Year 2018
Date Published 5/18/2018
Volume : Pages 360 : 758 - 763
PubMed Reference 29622724
Abstract Our understanding of kidney disease pathogenesis is limited by an incomplete
molecular characterization of the cell types responsible for the organ's
multiple homeostatic functions. To help fill this knowledge gap, we
characterized 57,979 cells from healthy mouse kidneys by using unbiased
single-cell RNA sequencing. On the basis of gene expression patterns, we infer
that inherited kidney diseases that arise from distinct genetic mutations but
share the same phenotypic manifestation originate from the same differentiated
cell type. We also found that the collecting duct in kidneys of adult mice
generates a spectrum of cell types through a newly identified transitional cell.
Computational cell trajectory analysis and in vivo lineage tracing revealed that
intercalated cells and principal cells undergo transitions mediated by the Notch
signaling pathway. In mouse and human kidney disease, these transitions were
shifted toward a principal cell fate and were associated with metabolic

Investigators with authorship
Katalin SusztakUniversity of Pennsylvania