| Authors |
Gallego-Perez D, Pal D, Ghatak S, Malkoc V, Higuita-Castro N, Gnyawali S, Chang
L, Liao WC, Shi J, Sinha M, Singh K, Steen E, Sunyecz A, Stewart R, Moore J,
Ziebro T, Northcutt RG, Homsy M, Bertani P, Lu W, Roy S, Khanna S, Rink C,
Sundaresan VB, Otero JJ, Lee LJ, Sen CK
|
| Submitted By |
Daniel Gallego-Perez on 2/19/2018 |
| Status |
Published |
| Journal |
Nature nanotechnology |
| Year |
2017 |
| Date Published |
|
| Volume : Pages |
12 : 974 - 979 |
| PubMed Reference |
28785092 |
| Abstract |
Although cellular therapies represent a promising strategy for a number of
conditions, current approaches face major translational hurdles, including
limited cell sources and the need for cumbersome pre-processing steps (for
example, isolation, induced pluripotency). In vivo cell reprogramming has the
potential to enable more-effective cell-based therapies by using readily
available cell sources (for example, fibroblasts) and circumventing the need for
ex vivo pre-processing. Existing reprogramming methodologies, however, are
fraught with caveats, including a heavy reliance on viral transfection.
Moreover, capsid size constraints and/or the stochastic nature of status quo
approaches (viral and non-viral) pose additional limitations, thus highlighting
the need for safer and more deterministic in vivo reprogramming methods. Here,
we report a novel yet simple-to-implement non-viral approach to topically
reprogram tissues through a nanochannelled device validated with
well-established and newly developed reprogramming models of induced neurons and
endothelium, respectively. We demonstrate the simplicity and utility of this
approach by rescuing necrotizing tissues and whole limbs using two murine models
of injury-induced ischaemia.
|
|
|