Mast cells and histamine are triggering the NF-?B-mediated reactions of adult
and aged perilymphatic mesenteric tissues to acute inflammation.
Authors Nizamutdinova IT, Dusio GF, Gasheva OY, Skoog H, Tobin R, Peddaboina C,
Meininger CJ, Zawieja DC, Newell-Rogers MK, Gashev AA
Submitted By Cynthia Meininger on 2/28/2017
Status Published
Journal Aging
Year 2016
Date Published
Volume : Pages 8 : 3065 - 3090
PubMed Reference 27875806
Abstract This study aimed to establish mechanistic links between the aging-associated
changes in the functional status of mast cells and the altered responses of
mesenteric tissue and mesenteric lymphatic vessels (MLVs) to acute inflammation.
We used an in vivo model of acute peritoneal inflammation induced by
lipopolysaccharide treatment of adult (9-month) and aged (24-month) F-344 rats.
We analyzed contractility of isolated MLVs, mast cell activation, activation of
nuclear factor-?B (NF-?B) without and with stabilization of mast cells by
cromolyn or blockade of all types of histamine receptors and production of 27
major pro-inflammatory cytokines in adult and aged perilymphatic mesenteric
tissues and blood. We found that the reactivity of aged contracting lymphatic
vessels to LPS-induced acute inflammation was abolished and that activated mast
cells trigger NF-?B signaling in the mesentery through release of histamine. The
aging-associated basal activation of mesenteric mast cells limits acute
inflammatory NF-?B activation in aged mesentery. We conclude that proper
functioning of the mast cell/histamine/NF-?B axis is necessary for reactions of
the lymphatic vessels to acute inflammatory stimuli as well as for interaction
and trafficking of immune cells near and within the collecting lymphatics.

Investigators with authorship
Cynthia MeiningerTexas A&M Health Science Center