Aberrant water homeostasis detected by stable isotope analysis.
Authors O'Grady SP, Wende AR, Remien CH, Valenzuela LO, Enright LE, Chesson LA, Abel ED,
Cerling TE, Ehleringer JR
Submitted By Submitted Externally on 3/4/2015
Status Published
Journal PLoS ONE
Year 2010
Date Published
Volume : Pages 5 : e11699
PubMed Reference 20657736
Abstract While isotopes are frequently used as tracers in investigations of disease
physiology (i.e., 14C labeled glucose), few studies have examined the impact
that disease, and disease-related alterations in metabolism, may have on stable
isotope ratios at natural abundance levels. The isotopic composition of body
water is heavily influenced by water metabolism and dietary patterns and may
provide a platform for disease detection. By utilizing a model of streptozotocin
(STZ)-induced diabetes as an index case of aberrant water homeostasis, we
demonstrate that untreated diabetes mellitus results in distinct combinations,
or signatures, of the hydrogen (delta2H) and oxygen (delta18O) isotope ratios in
body water. Additionally, we show that the delta2H and delta18O values of body
water are correlated with increased water flux, suggesting altered blood
osmolality, due to hyperglycemia, as the mechanism behind this correlation.
Further, we present a mathematical model describing the impact of water flux on
the isotopic composition of body water and compare model predicted values with
actual values. These data highlight the importance of factors such as water flux
and energy expenditure on predictive models of body water and additionally
provide a framework for using naturally occurring stable isotope ratios to
monitor diseases that impact water homeostasis.

Investigators with authorship
E. Dale AbelUniversity of Iowa
Adam WendeUniversity of Alabama at Birmingham