Sign-up for our newsletter
Protocols & Methods
Reagents & Resources
IMPC / KOMP Data
mTORC1-independent reduction of retinal protein synthesis in type 1 diabetes.
Fort PE, Losiewicz MK, Pennathur S, Jefferson LS, Kimball SR, Abcouwer SF,
Submitted Externally on 6/2/2014
Volume : Pages
63 : 3077 - 3090
Poorly controlled diabetes has long been known as a catabolic disorder with
profound loss of muscle and fat body mass due to a simultaneous reduction in
protein synthesis and enhanced protein degradation. By contrast, retinal
structure is largely maintained during diabetes in spite of reduced Akt activity
and enhanced cell death. Therefore, we hypothesized that retinal protein
turnover is regulated differently than other insulin-sensitive tissues such as
skeletal muscle. Ins2(Akita) diabetic mice and streptozotocin-induced diabetic
rats exhibited marked reductions of retinal protein synthesis matched by
concomitant reduction of retinal protein degradation, associated with preserved
retinal mass and protein content. The reduction in protein synthesis was
dependent on both hyperglycemia and insulin deficiency, but protein degradation
was only reversed by normalization of hyperglycemia. The reduction in protein
synthesis was associated with diminished protein translation efficiency but
surprisingly not with reduced activity of the mTORC1/S6K1/4E-BP1 pathway.
Instead, diabetes induced a specific reduction of mTORC2 complex activity. These
findings reveal distinctive responses of diabetes-induced retinal protein
turnover compared to muscle and liver that may provide new means to ameliorate
Investigators with authorship
University of Michigan-Ann Arbor
Neuropathy & Neurocognition
Welcome to the DiaComp Login / Account Request Page.
Note: Passwords are case-sensitive.
Please save my Email Address on this machine.
Not a member?
If you are a funded DiaComp investigator, a member of an investigator's lab,
or an External Scientific Panel member to the consortium, please
request an account.
Forgot your password?
Enter your Email Address and
There was a problem with the page:
Safari Browser Detected...
We strive to make the DiaComp site compatable with as many browsers as possible, but some of our third party tools don't work with the Safari browser.
In order to explore this site we highly recommend using the most recent versions of the following browsers:
© 2018 DiaComp.org
All rights reserved
Please acknowledge all posters, manuscripts or scientific materials that were generated in part or whole using funds from the Diabetic Complications Consortium(DiaComp) using the following text:
Financial support for this work provided by the NIDDK Diabetic Complications Consortium (RRID:SCR_001415, www.diacomp.org), grants DK076169 and DK115255
Citation text and image have been copied to your clipboard. You may now paste them into your document. Thank you!