Noninvasive imaging of retinal morphology and microvasculature in obese mice
using optical coherence tomography and optical microangiography.
Authors Zhi Z, Chao JR, Wietecha T, Hudkins KL, Alpers CE, Wang RK
Submitted By Charles Alpers on 3/17/2014
Status Published
Journal Investigative ophthalmology & visual science
Year 2014
Date Published 2/1/2014
Volume : Pages 55 : 1024 - 30
PubMed Reference 24458155
Abstract To evaluate early diabetes-induced changes in retinal thickness and
microvasculature in a type 2 diabetic mouse model by using optical coherence
tomography (OCT)/optical microangiography (OMAG)., Twenty-two-week-old obese
(OB) BTBR mice (n = 10) and wild-type (WT) control mice (n = 10) were imaged.
Three-dimensional (3D) data volumes were captured with spectral domain OCT using
an ultrahigh-sensitive OMAG scanning protocol for 3D volumetric angiography of
the retina and dense A-scan protocol for measurement of the total retinal blood
flow (RBF) rate. The thicknesses of the nerve fiber layer (NFL) and that of the
NFL to the inner plexiform layer (IPL) were measured and compared between OB and
WT mice. The linear capillary densities within intermediate and deep capillary
layers were determined by the number of capillaries crossing a 500-µm line. The
RBF rate was evaluated using an en face Doppler approach. These quantitative
measurements were compared between OB and WT mice., The retinal thickness of the
NFL to IPL was significantly reduced in OB mice (P < 0.01) compared to that in
WT mice, whereas the NFL thickness between the two was unchanged. 3D
depth-resolved OMAG angiography revealed the first in vivo 3D model of mouse
retinal microcirculation. Although no obvious differences in capillary vessel
densities of the intermediate and deep capillary layers were detected between
normal and OB mice, the total RBF rate was significantly lower (P < 0.05) in OB
mice than in WT mice., We conclude that OB BTBR mice have significantly reduced
NFL-IPL thicknesses and total RBF rates compared with those of WT mice, as
imaged by OCT/OMAG. OMAG provides an unprecedented capability for
high-resolution depth-resolved imaging of mouse retinal vessels and blood flow
that may play a pivotal role in providing a noninvasive method for detecting
early microvascular changes in patients with diabetic retinopathy.

Investigators with authorship
Charles AlpersUniversity of Washington