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Publication
Considerations for quantification of lipids in nerve tissue using
matrix-assisted laser desorption/ionization mass spectrometric imaging.
Authors
Landgraf RR, Garrett TJ, Conaway MC, Calcutt NA, Stacpoole PW, Yost RA
Submitted By
Nigel Calcutt on 1/30/2013
Status
Published
Journal
Rapid communications in mass spectrometry : RCM
Year
2011
Date Published
10/30/2011
Volume : Pages
25 : 3178 - 3184
PubMed Reference
21953974
Abstract
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometric imaging
is a technique that provides the ability to identify and characterize endogenous
and exogenous compounds spatially within tissue with relatively little sample
preparation. While it is a proven methodology for qualitative analysis, little
has been reported for its utility in quantitative measurements. In the current
work, inherent challenges in MALDI quantification are addressed. Signal response
is monitored over successive analyses of a single tissue section to minimize
error due to variability in the laser, matrix application, and sample
inhomogeneity. Methods for the application of an internal standard to tissue
sections are evaluated and used to quantify endogenous lipids in nerve tissue. A
precision of 5% or less standard error was achieved, illustrating that MALDI
imaging offers a reliable means of in situ quantification for microgram-sized
samples and requires minimal sample preparation.
Investigators with authorship
Name
Institution
Nigel Calcutt
University of California San Diego
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Please acknowledge all posters, manuscripts or scientific materials that were generated in part or whole using funds from the Diabetic Complications Consortium(DiaComp) using the following text:
Financial support for this work provided by the NIDDK Diabetic Complications Consortium (RRID:SCR_001415, www.diacomp.org), grants DK076169 and DK115255
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