Minimally invasive aortic banding in mice: Effects of altered cardiomyocyte
insulin signaling during pressure-overload.
Authors Hu P, Zhang D, Swenson L, Chakrabarti G, Abel ED, Litwin SE.
Submitted By E. Dale Abel on 6/9/2003
Status Published
Journal American journal of physiology. Heart and circulatory physiology
Year 2003
Date Published 9/1/2003
Volume : Pages 285 : H1261 - H1269
PubMed Reference 12738623
Abstract We have developed a minimally invasive method for producing left ventricular
pressureoverload in mice. Using this technique we quickly and reproducibly
banded the transverse aorta with low surgical morbidity and mortality. Minimally
invasive transverse aortic banding (MTAB) acutely and chronically increased left
ventricular (LV) systolic pressure, increased heart weight/body weight and
induced myocardial fibrosis. We used this technique to determine
whether reduced insulin signaling in the heart altered the cardiac response to
pressure-overload. Mice with cardiac myocyte restricted knock out of the insulin
receptor (CIRKO) have smaller hearts than wild type (WT) controls. Four weeks
after MTAB, WT and CIRKO mice had
comparably increased LV systolic pressure, increased cardiac mass and induction
of mRNA for â-myosin heavy chain and atrial natriuretic factor. However, CIRKO
hearts were more dilated, had depressed left ventricular systolic function by
echocardiography and had greater interstitial
fibrosis than WT. Expression of connective tissue growth factor was increased in
banded CIRKO hearts compared to WT. Thus, lack of insulin signaling in the heart
accelerates the transition to a more decompensated state during cardiac
pressure-overload. The use of the MTAB approach
should facilitate the study of the pathophysiology and treatment of
pressure-overload hypertrophy.

Investigators with authorship
E. Dale AbelUniversity of Iowa