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IMPC / KOMP Data
Research Assistant Professor
Johns Hopkins University School of Medicine
A novel AI-driven approach to image and interrogate 3D histology of gastric tissues diabetic gastroparesis patients
Gastroparesis, a condition that causes significant slowing of gastric motility, nausea, and abdominal pain, is a significant complication in diabetic patients. While gastrointestinal motility is regulated by the Enteric Nervous System (ENS) in conjunction with associated cells, a clear understanding of how the ENS is impacted in diabetic gastroparesis patients is lacking. While prior data suggests significant reduction in nerve fiber staining in the gastric tissue from patients, there is no clarity on whether neuronal numbers, their networks, or their proximity to associated cells such as ICC and macrophages are pathologically impacted in disease. This gap, which exists since neuronal networks exist in three-dimensional (3D) space which 2 dimensional cross-sections of tissue cannot capture accurately, has stymied our efforts in understanding whether and how structural changes to the ENS should be quantified for assessing pathology, and whether future therapeutic interventions should target the ENS for normalizing gastric function. It is with this rationale that in this proposal, we aim to create a 3D high-resolution and high-information map of the full thickness gastric tissues from control and diabetic gastroparesis patients to tease out how the ENS and their associated cells significant for gastric motility are altered with disease. Using our novel software algorithms CODA and VAMPIRE, we will construct 3D images of neurons, macrophages, ICC, and smooth muscle from 2D immunostained tissue sections, and assess disease associated changes to neuronal structure, cell-cell proximity, and cell health. Further, these images can be analyzed by other artificial intelligence-driven approaches in the future to learn how diabetes impacts gastric tissue structure.
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The DiaComp Steering Committee is the governing body of the consortium. The principle function of this committee is to guide the scientific direction of the consortium. This is accomplished by creating various subcommittees necessary to advance the scientific goals and providing guidance to the broader complications research community. Policies for the consortium are developed through consultation with the
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The DiaComp Gastro-Intestinal (GI) Committee has the principal function of furthering the mission of the consortium with regard to diabetic gastro-intestinal (GI) and liver disease
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Please acknowledge all posters, manuscripts or scientific materials that were generated in part or whole using funds from the Diabetic Complications Consortium(DiaComp) using the following text:
Financial support for this work provided by the NIDDK Diabetic Complications Consortium (RRID:SCR_001415, www.diacomp.org), grants DK076169 and DK115255
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