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DiaComp Funded Abstracts

Program Application Abstract
The administration of NAD+ precursor, Nicotinamide Riboside Chloride, may serve to improve mitochondrial function.
Woodford, Yushekia   (University of Washington)
Mitochondrial dysfunction plays a significant role in the pathogenesis of heart disease and diabetes. Several studies in patients with heart disease and diabetes have shown significant defects in mitochondrial function in peripheral mononuclear cells (PBMCs). This supports their potential for monitoring the disease process and therapeutic intervention. NAD+ precursor, Nicotinamide Riboside Chloride (NR), is a useful tool to indirectly regulate and enhance oxidative metabolism. We hypothesize that NR supplementation may improve mitochondrial function, heart disease, and even restore patient heart function. To test this hypothesis, we screened and enrolled patients with heart disease into the NR study and collected 10 mL of blood the patients. SepMate was used to isolate PBMCs and the Seahorse instrument was used to monitor mitochondrial function. Additionally, PBMCs from a group of healthy people were treated with 1mM NR from ChromaDex NIAGEN for 4 hrs at 37°C in a CO2 incubator. After 12 weeks of NR supplementation, another 10 mL of blood will be drawn from patients in the study. Maximal respiration oxygen consumption rate was higher in the group of healthy people. PBMCs from the patients with heart disease had a lower maximal respiration compared to the group of healthy people. PBMCs treated with 1mM NR for 4 hrs had an increase in OCR during maximal respiration. Information gathered from the beginning stages of this study suggest that patients with heart disease have decreased mitochondrial function as depicted by the decrease in maximal respiration. The results from the PBMCs treated with 1mM NR show a possible correlation between NR and increased maximal respiration. This may suggest NR as a means to increase maximal respiration and possibly improve mitochondrial function.