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DiaComp Funded Abstracts

Program Application Abstract
Neurostructural morphology in CNS-­specific RPGRIP1L deficient mice
Wiese, Mohammad   (Columbia University in the City of New York)
Primary cilia are important cellular structures involved in mediating cell signaling involved in processes such as proliferation, apoptosis, and differentiation. Perturbation of ciliary components have been shown to cause obesity in both humans and mice. Mice with a systemic hypomorphism of Rpgrip1l, a component of the ciliary transition zone, exhibit increased food intake, leading to increased adiposity. In our current study, we found that deletion of this ciliary component induced in the central nervous system of adult mice also leads to increased food intake and increased adiposity. In order to further characterize these mice on the molecular level, we attempted to study the morphology of primary cilia in the arcuate nucleus of the hypothalamus, an area thought to play an important role in the regulation of energy homeostasis. To do so, brains were harvested from Rpgrip1l mutant and control mice and immunohistochemically processed for primary cilia. We utilized specialized computer software to find that while the number of ciliated cells was decreased in the arcuate nucleus of the hypothalamus, ciliary morphology appeared unchanged. Moreover, we noticed a decrease in the total number of cells, suggesting that the decrease in the number of arcuate cilia of CNS-­ specific Rpgrip1l deleted mice may be due to a decrease in the total number of cells in the arcuate hypothalamus upon. This finding suggests that Rpgrip1l may play an important role in the structural integrity of hypothalamic neurocircuitry involved in energy homeostasis.