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Mouse Diets



Protocols


The following protocols are used by the DiaComp. Click the title for details, or download the protocol in PDF format (if available).


Complications:  (Click an image below to filter by diabetic complication)

Cardiovasculary/Cardiomyopathy Cardiomyopathy
Cardiovascular
Neuropathy Neuropathy Wound-Healing Wound
Healing
Gastro-Intestinal Gastro-Intestinal
(GI)
Pediatric Endocrinology Pediatric
Endocrinology
Retinopathy Retinopathy
Nephropathy Nephropathy Uropathy Uropathy All_Complications Show All
Complications


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BreedingContactComplication
Breeding NONcNZO10/LtJ (NON/NZ) and NON/ShiLtJ (NON/Shi) mice
      
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Breeding Scheme and Selection of Animals for DCC Experiments
     
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Complication InductionContactComplication
Induction of Type 1 Diabetes, Myocardial Infarction, Echocardiography, Measurement of Blood Glucose and Tissue Weights
 
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Diabetes InductionContactComplication
Ablation of ß-cells in Tg(ins:NTR-mCherry) transgenic zebrafish using metronidazole
 
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Dechorionation of zebrafish embryos with Pronase for metronidazole-mediated ß-cell ablation
 
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High Dose STZ Induction Protocol
      
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Low-Dose Streptozotocin Induction Protocol (mouse)
      
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Mouse Diets Used in Diabetic Complication Research

DissectionContactComplication
Isolation of rodent eyes for analysis of diabetic retinopathy
 
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Removal and Preservation of the Urinary Bladder
 
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HistologyContactComplication
En face preparation and quantification of aortic surface area covered by atherosclerotic lesions
 
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Mouse dissection and preparation of heart (aortic sinus) and brachiocephalic artery for lesion quantitation by sectioning
 
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PCR AmplificationContactComplication
Quantitative PCR analysis to assess gene expression changes in hyperglycemic larval zebrafish
 
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Phenotype AssayContactComplication
Aortic Banding in Mice
 
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Atherosclerotic Morphometry in Pigs
 
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Cardiac AMPK Assay
 
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CATALASE
      
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Confocal imaging of live larval zebrafish for assessing peripheral neuropathy
 
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Conscious Cystometry Bladder Function Testing
 
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Creatinine Clearance by HPLC
 
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Creatinine Companion Protocol Assay (Exocell)
 
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Determination of Glomerular Filtration Rate in Conscious Mice using FITC-inulin
 
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Echocardiography: Mouse
 
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Evaluation of Mitochondrial Function
 
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Frequently sampled intravenous glucose tolerance test
     
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Glucose
      
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Glucose uptake in isolated cardiomyocytes.
 
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Histological Evaluation of Renal Fibrosis in Mice
 
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Intraperitoneal Glucose Tolerance Testing (IPGTT)
      
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Isoproterenol
 
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Lipid Profiles in Mice
 
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Lipoprotein separation by FPLC
 
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Measurement of Left Ventricular Hemodynamic Parameters in Intact Mice
 
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Measurement of Left Ventricular Performance in Langendorff Perfused Mouse Hearts
 
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Mesangial Index Quantification
 
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Morphometry: Mouse
      
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NADH Oxidase Activity
      
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Nerve Conduction Velocity Tests
 
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Neuropathy Phentoyping Protocols
 
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Pancreatic Insulin Concentration
      
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Phenotypic Characterization of the Working Heart
 
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Plantar Analgesia Test for Hind Paw and Tail
 
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Plasma Insulin (LINCO ELISA)
      
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Podocyte Count and Density Analysis
 
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Prepare Plantar Footskin for Morphometry
 
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Preparing Nerves for Morphometry
 
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Quantification of atherosclerosis at the aortic sinus
 
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Quantitative Magnetization Transfer MR Imaging (qMT-MRI) of Diabetic Mouse Kidney
 
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Serum, Blood and Body Weight Measurements in Pigs
      
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Tail Flick
 
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Thiobarbituric acid reactive substances
 
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TRAP
      
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Ultra Sensitive Rat Insulin ELISA Kit (Crystal Chem Inc)
      
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Ultracentrifugal separation of HDL alone and calculation of non-HDL
 
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Ultracentrifugal separation of VLDL, LDL and HDL
[Consortial]
      
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Urine Albumin ELISA (Albuwell M kit)
 
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Western Analysis used in Oxidative Stress Protocols
 
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StainingContactComplication
aldehyde fuchsin
     
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aldehyde fuchsin/H&E
     
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Fouchet's stain
     
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H&E Staining
[Consortial]
     
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Lead citrate-uranyl acetate
[Consortial]
     
Masson's trichrome
     
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MOVAT Pentachrome Staining
     
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Periodic acid Schiff (PAS)
     
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Silver Methenamine
     
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Toluidine blue
[Consortial]
     

TechniqueContactComplication
Determination of Podocyte Number and Density in Rodent Glomeruli
 
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Induce Stroke via Hypoxia-Ischemia
  
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Restoration of euglycemia in the RCS10 mice with Metformin
      
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Vascular perfusion of mice
     
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WT-1 Staining Protocol for Podocytes
 
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Validation CriteriaContactComplication
Validation of Mouse Models of Diabetic Cardiovascular Disease
[Consortial]
 
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Validation of Mouse Models of Diabetic Nephropathy
[Consortial]
 
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